• Bpod

  • Deliver air puf by sending TTL from RP2.1

Hi,

I want to send TTL from RP2.1 to Bpod to deliver air puff via the solenoid valve. My first question I have to connect the LAN cable between airpuf delivery system and behavioral ports or I have to connect the LAN cable between airpuf delivery system and module ports. Also For controlling the Bpod via TTL to deliver airpuf I can use GUI or I have to write own code? Is there any an example script to get idea?

Best Regards,
Saleh

Hello Saleh,

You need the RP2.1 to output a 5V pulse. This can come as a digital signal from the 'Digital I/O' DSUB connector, or as an analog 5V signal from the 'Out1' or 'Out2' BNC connector in the 'Analog I/O' section.

To connect the RP2.1's analog output to the Bpod State Machine's digital input, all you'd need is a BNC cable. If you have a wire adapter for the RP2.1's digital DSUB outputs, you'd patch the signal and ground to the 'S' and 'GND' pins on the right side of the port interface board as shown. You'd then connect an Ethernet cable between the port interface board and one of the state machine's 'Behavior Port' channels.

You could then construct a state machine description with a first state that waits for the TTL, and a second state that opens the valve for your desired amount of time.

Please let me know if this works for you.

Thanks,
Josh

    Dear Josh,

    Thank you. My paradigm is delivering air puf and also play tune sound to mice at the same time. It is easer to control Bpod by Rp2 or control Rp2 by Bpod to design this paradigm?
    Is there any example script to get an iead?

    Best Regards

    Hello Saleh,

    If you have the Bpod HiFi module, you can eliminate the RP2.1 entirely. Unfortunately I do not have example scripts that interface Bpod with RP2.1.

    -Josh

      Josh
      Dear Josh,
      Thank you. I setup Rp2 and now it sends TTL to Bpod. I didn't find examples which command I have to write in Bpod for first state that wait for TTL. Could you please advise me about this.

      Best Regards,
      Saleh

        Hello Saleh,

        You can start with the one-state example here.
        In the StateChangeConditions section, add:

        • {'BNC1High', MyNextState} if the RP2.1 is patched to a BNC input channel
        • {'Port1In', MyNextState} if the RP2.1 is patched to a port input channel

        You don't need to install anything special to use the valves. In the OutputActions section, add:
        {'Valve2', 1} to open valve 2

        I hope this helps,
        Josh

        Thank you Josh,

        I want to generate sound with Rp2 (with 2 sec pulse width) and send TTL to Bpod for delivering airpuf (for 1 sec) for each sound pulse. Could you please check this script is fine or not?

        function Airpuf
        sma = NewStateMachine;

        sma = AddState(sma, 'Name', 'TTL1', ...
        'Timer', 0,...
        'StateChangeConditions', {'BNC1High', Delay},...
        'OutputActions', {'Valve2', 1});
        sma = AddState(sma, 'Name', 'Delay', ...
        'Timer', 1,...
        'StateChangeConditions', {'BNC1High', 'end'},...
        'OutputActions', {'Valve2', 1});

        SendStateMatrix(sma);
        RawEvents = RunStateMatrix;
        end

        How does the RP2 know when to play the sound?

        The code you wrote opens valve 2 immediately, because {'Valve2', 1} is in the output actions of the first state.
        Output actions are executed on entering a state.
        The second state also has {'Valve2', 1} so the valve would remain open until a second TTL pulse arrives from RP2, ending the trial.

        -Josh

          Josh
          Thank you Josh,

          I defined by Pulsedtrain in RPvdsx. I changed script. Is it correct?
          In this script solenoid deliver puf until get TTL or finished in first pulse and I have to consider loop in my script?

          function Airpuf
          sma = NewStateMachine;

          sma = AddState(sma, 'Name', 'Openvalve', ...
          'Timer', 0.1,...
          'StateChangeConditions', {'BNC1High', Closevalve},...
          'OutputActions', {'Valve2', 1});
          sma = AddState(sma, 'Name', 'Closevalve', ...
          'Timer', 1,...
          'StateChangeConditions', {'BNC1High', 'end'},...
          'OutputActions', {'Valve2', 2});

          SendStateMatrix(sma);
          RawEvents = RunStateMatrix;
          end

            Hello Saleh,

            If you want to close the valve in the 'Closevalve' state, you'd use:
            {'Valve2', 0}
            or leave it blank, as the default state is closed.

            -Josh

              Dear Josh,

              I ran above protocol and faced these errors. Could you please advise me about that.
              thank you

              Error using SendStateMachine
              Please define the following states using the AddState function before sending the state machine: end

              Error in SendStateMatrix (line 2)
              Confirmed = SendStateMachine(sma);

              Error in Airpuf (line 13)
              SendStateMatrix(sma);

              Error in run (line 91)
              evalin('caller', strcat(script, ';'));

              Error in LaunchManager>launch_protocol (line 843)
              run(protocolPath);

              Error while evaluating DestroyedObject Callback.

              To exit the trial, in StateChangeConditions, you should use '>exit', not 'end'

              -Josh

                Hello Josh,

                I run Bpod with this protocol but it delivers airpuf continuously until I stop Bpod. Could you please advise me about that.

                Best Regards,
                Saleh

                Hello Saleh,

                Can you please share the updated version of your code?

                Thanks,
                Josh

                My last script:

                function Airpuf
                sma = NewStateMachine;

                sma = AddState(sma, 'Name', 'Openvalve', ...
                'Timer', 0.1,...
                'StateChangeConditions', {'BNC1High', Closevalve},...
                'OutputActions', {'Valve2', 1});
                sma = AddState(sma, 'Name', 'Closevalve', ...
                'Timer', 1,...
                'StateChangeConditions', {'BNC1High', 'exit'},...
                'OutputActions', {'Valve2', 2});

                SendStateMatrix(sma);
                RawEvents = RunStateMatrix;
                end

                When I run Bpod the icon of BNC1 in Bpod console is not green and I think Bpod didn't identify input TTL. I also turned off RP2. I run the code again and Bpod opened valve without input TTL signal untill I stop Bpod.

                Best Regards,
                Saleh

                Hello Saleh,

                Output actions are set when you enter a state. You need to remove {'Valve2', 1} from the output actions of your first state.

                Also, the syntax is {'Valve2', 1} to open the valve, and {'Valve2', 0} to close it. {'Valve2', 2} isn't a valid instruction. In your 'CloseValve' state, change 2 to 0.

                Please let me know if this works for you.

                Thanks,
                Josh

                  Josh

                  Sorry my last script was:

                  My last script:

                  function Airpuf
                  sma = NewStateMachine;

                  sma = AddState(sma, 'Name', 'Openvalve', ...
                  'Timer', 0.1,...
                  'StateChangeConditions', {'BNC1High', Closevalve},...
                  'OutputActions', {'Valve2', 1});
                  sma = AddState(sma, 'Name', 'Closevalve', ...
                  'Timer', 1,...
                  'StateChangeConditions', {'BNC1High', 'exit'},...
                  'OutputActions', {'Valve2', 0});

                  SendStateMatrix(sma);
                  RawEvents = RunStateMatrix;
                  end

                  for first state which command I have to replace with {'Valve 2',1}? Previously (in post 6) you told me In the OutputActions section, add:
                  {'Valve2', 1} to open valve 2

                  Best Regards