GlobalTimerTrig

Oly

I have a question
I want to set two GlobalTimer to count separately. However, I found that when I from the state 'CountForLeftLick_FirstLick' enter the state 'CountForLeftLick', not only does GlobalTImer1 start counting (eg. timestamp is 1.0001), but GlobalTimer2 also starts counting (eg. timestamp is 1.0002). Does anyone konw how to solve this issue? If so, please help me. Thanks!!!

The specific code is as follows:
sma = SetGlobalTimer(sma, 'TimerID',1,'Duration', S.GUI.WindowForLickTimes);
sma = SetGlobalTimer(sma, 'TimerID',2,'Duration', S.GUI.WindowForLickTimes);
sma = AddState(sma,'Name','CountForLeftLick_FirstLick', ...
'Timer', 0,...
'StateChangeConditions', {'Tup','CountForLeftLick'},...
'OutputActions', {'GlobalTimerTrig',1});
sma = AddState(sma,'Name','CountForRightLick_FirstLick', ...
'Timer', 0,...
'StateChangeConditions', {'Tup','CountForRightLick'},...
'OutputActions', {'GlobalTimerTrig',2});

Josh

Hello Oly,

I tried the code you shared, but I wasn't able to replicate the issue.
Here's the code, formatted so that it can be executed by itself with Bpod open:

`WindowForLickTimes = 0.5;

sma = NewStateMachine;

sma = SetGlobalTimer(sma, 'TimerID',1,'Duration', WindowForLickTimes);
sma = SetGlobalTimer(sma, 'TimerID',2,'Duration', WindowForLickTimes);
sma = AddState(sma,'Name','CountForLeftLick_FirstLick', ...
'Timer', 0,...
'StateChangeConditions', {'Tup','CountForLeftLick'},...
'OutputActions', {'GlobalTimerTrig',1});

sma = AddState(sma,'Name','CountForRightLick_FirstLick', ...
'Timer', 0,...
'StateChangeConditions', {'Tup','CountForRightLick'},...
'OutputActions', {'GlobalTimerTrig',2});

sma = AddState(sma,'Name','CountForLeftLick', ...
'Timer', 1,...
'StateChangeConditions', {'Tup','>exit'},...
'OutputActions', {});

sma = AddState(sma,'Name','CountForRightLick', ...
'Timer', 1,...
'StateChangeConditions', {'Tup','>exit'},...
'OutputActions', {});

SendStateMachine(sma);
RawEvents = RunStateMachine;
TE = struct;
TE = AddTrialEvents(TE, RawEvents);
disp(TE.RawEvents.Trial{1}.Events)`

The output I see is:

GlobalTimer1_Start: 1.0000e-04 Tup: [1.0000e-04 1.0001] GlobalTimer1_End: 0.5000

Please let me know if you see something different.

Thanks,
Josh

Oly

Thanks for your reply. I have solved this problem by adding an additional state (e.g. GlobalTimer1
_Trig) in my code (I did not show the other part of my code).

I have another question: how to trigger two valves simultaneously. When I use 'ValveState' in 'OutputActions', I only can trigger one valve.

Josh

Hi Oly,

I'm glad that worked!

To trigger multiple valves, the value of 'ValveState' is an integer that encodes the state of the valves in binary, e.g.
1 (binary 0001), Valve 1 open, all others closed
3 (binary 0011), Valves 1+2 open, all others closed
4 (binary 0100), Valve 3 open, all others closed

Please let me know if this works for you.

Thanks,
Josh

Oly

Josh
Hi Josh,

If I want to open valve 1+4 simultaneously, the binary should be '1001', and the corresponding number is '9', so I should write the code as following:
sma = AddState(sma, 'Name', 'RightReward', ...
'Timer', ValveTime_Short,... %
'StateChangeConditions', {'Tup', 'ITI'},...
'OutputActions', {'ValveState', 9});

Is this correct?

Oly

Hi Josh,

Thanks for your reply.

I want to confirm that each number indicates one valve, and the sequence of valve is from the right to left, not from the left to right. In addition, '0' is 'off' and '1' is 'on'.

Josh

Hi Oly,

Correct. So to open valves 1 and 2 you'd use:
{'ValveState', 3}

Tip: To convert between integers and binary in MATLAB you can use the dec2bin() and bin2dec() functions.

-Josh

Josh

Yes, that's correct!

Oly

Josh

Thank you, very much!!!

Oly

Josh
If I want to start two GlobalTimer simultaneously, is it the same as valve?

Josh

No, for 'GlobalTimerTrig', integers are timer indexes. To trigger more than 1, you'd use a binary string:
{'GlobalTimerTrig', '1001'} % Trigger timers 1 and 4

Josh

Oly

Josh
The sequence of global timer is also from the right to left?
If I set two global timers, and want to open global timer 2, the code should be {'GlobalTimerTrig','01'}?
If I set four global timers, and want to open global timer 2, the code should be {'GlobalTimerTrig','0010'}?

Josh

Hi Oly,

The values are both correct. The length of the binary string isn't important - it is fed to MATLAB's bin2dec() function, which disregards leading zeros.

Best,
Josh

Oly

Josh
Thank you , very much !!!

I have another question about HiFi module. I write the code as following, but I can't hear the sound, I don't know why it doesn't work.

sf = 192000; % Use max supported sampling rate
H.SamplingRate = sf;
GoSound = GenerateSineWave(sf, S.GUI.SinWaveFreqGo, S.GUI.SoundDuration)*.9;
H.HeadphoneAmpEnabled = true; H.HeadphoneAmpGain = 10; % Ignored if using HD version of the HiFi module
H.DigitalAttenuation_dB = 0; % Set a negative value here if necessary for digital volume control.
H.load(1, GoSound);
envelope = 1/(sf*0.001):1/(sf*0.001):1;
H.AMenvelope = envelope;

sma = AddState(sma,'Name','SessionStart', ...
                'Timer', 5,...
                'StateChangeConditions', {'Tup','SoundStimulus'},... 
                'OutputActions', {'HiFi1','*'}); 
sma = AddState(sma,'Name','SoundStimulus', ...
                'Timer', S.GUI.SoundDuration,...
                'StateChangeConditions', {'Tup','LickDelay'},...
                'OutputActions',  {'HiFi1', ['P' 0], 'BNCState', 1});

Josh

Hello Oly,

The code looks good to me. What amplifier or amplified speakers are the HiFi module outputs connected to?

Thanks,
Josh

Oly

Josh
I have tried different speakers.
When I run the code from https://github.com/sanworks/Bpod_Gen2/tree/master/Examples/Protocols/Sound/HiFiSound2AFC, I can hear the sound, but when I run the code as above, I can't hear the sound, so I'm confused about it.

Josh

Hello Oly,

Can you please share the entire .m file you're trying to run?
If it's too big to paste (or multiple files), please send to support@sanworks.io

Thanks,
Josh

Oly

Josh
I have sent .m file to your email address. I want to express my sincere gratitude for your willingness to spend your time and effor helping me resolve this issue.

Thank you so much!!!

Josh

Hello Oly,

I didn't have TotalRewardDisplay_zha.m, so I commented out lines 79 and 258 where it is used.
I ran the protocol, and for me it works - the HiFi module plays a 300Hz tone after the SessionStart state.

This likely points to hardware.

Please try the following at the MATLAB command prompt with the Bpod console GUI open:

H = BpodHiFi(BpodSystem.ModuleUSB.HiFi1);
sf = 192000; % Use max supported sampling rate
H.SamplingRate = 192000;
GoSound = GenerateSineWave(192000, 1000, 1);
H.HeadphoneAmpEnabled = true; 
H.HeadphoneAmpGain = 10; % Ignored if using HD version of the HiFi module
H.DigitalAttenuation_dB = 0; 
H.load(1, GoSound);
H.push;
H.play(1);
pause(1);
clear H

You should hear a 1kHz tone play for 1 second.

Can you please share the make and model of the audio amplifiers and speakers you tried, and describe any cabling you may have used to connect them to the HiFi module?

Thanks,
Josh

Oly

Josh
Thanks for your time!
Actually, after multiple attempts, I have come to realize that the issue may not be wtih the code but with the hardware. Now, I connect the speaker, HiFi module, and state machine before connecting to the computer and running the Bpod, which seems to resolve the issule of not hearing sound. But I am confused why it has such a significant impact on whether I can hear the sound (I also connect these assemblies before, but the timing may after connecting to the computer).